Midbrain neurons expressing ChR2-eYFP (40x confocal image)
3-color image of the dorsal striatum. Neurons (blue) are surrounded by axon terminals expressing ChR2-eYFP (green). In this preparation, they co-localize with terminals stained for tyrosine hydroxylase (red), producing a mostly yellow overlay.
Midbrain neurons (blue). ChR2-eYFP expression (green) is restricted to tyrosine hydroxylase containing neurons (red), producing a mostly yellow overlay.
Neurons in the substantia nigra stained for tyrosine hydroxylase (red), expressing ChR2-eYFP from a retrograde viruse injected in the dorsal striatum.
Neurons in the dorsal prefrontal cortex (pink), retrogradely labeled with cholera toxin from the dorsal striatum.
Lateral orbitofrontal cortex neurons expressing ChR2-eYFP (green).
Neurons in the medial prefrontal cortex (purple) expressing GCaMPgf (green).
Dorsomedial striatum image. Neurons (yellow) surrounded by Arch-T3.0-eYFP expressing axon terminals (pink) projecting from the orbitofrontal cortex.
Axonal fibers expressing ChR2-eYFP (green) traversing anterior midbrain.
Whole brain coronal section image. Axon terminals from neurons in the substantia nigra in the dorsal striatum (green, left side).
Dorsal striatum image. Neurons (light blue) surrounded by axon terminals expressing ChR2-eYFP (yellow). Neurons co-labeled for c-fos (pink) were activated by an experimental manipulation.
Nucleus accumbens core image. Neurons (blue) surrounded by axon terminals expressing ChR2-eYFP (green) that overlap with terminals stained for tyrosine hydroxylase (red).
Whole brain coronal section image containing the midbrain. Dopaminergic neurons are shown with a stain for tyrosine hydroxylase (red). Neurons in the substantia nigra expressing ChR2-eYFP (green) from a infusion of a retrograde virus in the dorsal striatum.
Image of the ventral tegmental area in the midbrain. Neurons (blue) were co-transfected with viruses containing GCaMP6f (green) and ChrimsonR-tdTomato (red). In this preparation, they overlap with neurons stained for tyrosine hydroxylase (white).
Whole brain coronal section image. Axon terminals from neurons in the orbitofrontal cortex expressing ChR2-eYFP (green) in the thalamus and basolateral amygdala.
Orbitofrontal cortex neurons (dark blue) expressing ArchT3.0-eYFP (light blue).
Image of the medial ventral tegmental area. Dopaminergic neurons, expressing tyrosine hydroxylase (pink), co-express ChR2-eYFP (light blue) in this preparation.
